Figure 3.

Euchromatic location of the SSL gene. (A) In situ hybridization of the SSL-bearing cosmid 9 and of the 2.3-kb BamHI subclone to D. melanogaster and to D. simulans (Inset) polytene chromosomes, respectively. The 60D1–2 hybridization sites on chromosome 2 in both species are indicated by arrowheads. (B) Southern analysis of ≈3 μg of EcoRI-cleaved DNA from salivary glands (lane 1) and from whole flies (lane 2) probed with cDNA 911. (C) Southern analysis of 6 μg of female DNA (Batumi stock) digested with BamHI (lane 1), PstI (lane 2), and BglII (lane 3) and probed with cDNA 911. Approximate sizes of fragments are indicated in kilobases.