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. Author manuscript; available in PMC: 2008 Oct 24.
Published in final edited form as: Brain Res. 2007 Aug 22;1176:133–142. doi: 10.1016/j.brainres.2007.07.083

Fig. 6.

Fig. 6

Induction of late apoptosis [SR-VAD-FMK (+) 7-AAD (+)] in SH-SY5Y cells by rotenone (10 μM) alone, EGCG (25 μM) alone, or rotenone (10 μM) plus EGCG (25 μM). The cells were treated by the test compounds for 20 h before measuring apoptosis by flow cytometry. Cells undergoing late apoptosis were identified by positive staining with the fluorescent compounds, SR-VAD-FMK (which binds covalently to activated caspases) and with 7-amino-actinomycin D (7-AAD) which permeates later stage apoptotic and dying cells. The data are expressed as means ± S.E. of 4 experiments. #P < 0.05 versus control (Con) group. *P < 0.05, compared to rotenone- or EGCG-treated group.