Skip to main content
. Author manuscript; available in PMC: 2008 Oct 24.
Published in final edited form as: Brain Res. 2007 Aug 22;1176:133–142. doi: 10.1016/j.brainres.2007.07.083

Fig. 8.

Fig. 8

SDS-PAGE and Western blot of crude 10,000g-fractions of SH-SY5Y cells treated with rotenone and/or EGCG for 24 h. Twenty μg of protein were loaded into 12% Tris-HCl gel. The gel (panel 1) was stained with IEF Gel staining solution. The blot (panel 2) was incubated with anti-HNE IgG for detection of immunoreactive bands. A: DMSO, B: 10 μM rotenone, C: 25 μM EGCG, D: EGCG (25 μM) + rotenone (10 μM). The identified proteins in sample D that matched with the Western blot are; 1: Splicing factor, 2: Heat shock protein 90-β, 3: Heat shock protein 71, 4: not assigned, 5: not assigned, 6: β-Actin, 7: Nuclear ribonucleoprotein A3, 8: Nuclear ribonucleoprotein A2/B1, 9: Voltage-dependent anion-selective channel protein 1, 10: ADP/ATP translocase 3, 11: not assigned, 12: Gluthathione S-transferase P, 13: Histone H2B, H2AV, H3.1t, and 14: Histone H4.