Table 1.
Treatment | Hydrogen peroxide (μM)
|
|
---|---|---|
No pyruvate media | With 1.5 μM pyruvate media | |
Control | ND | ND |
Rotenone | ||
1 μM | ND | ND |
5 μM | ND | ND |
10 μM | ND | ND |
25 μM | ND | ND |
50 μM | ND | ND |
EGCG | ||
1 μM | ND | ND |
5 μM | ND | ND |
10 μM | 0.33 ± 0.04 | ND |
25 μM | 0.72 ± 0.04 | ND |
50 μM | 1.72 ± 0.10 | 0.87 ± 0.03* |
Rotenone (RT) + EGCG | ||
1 μM EGCG + 10 μM RT | ND | ND |
5 μM EGCG + 10 μM RT | 0.30 ± 0.04 | ND |
10 μM EGCG + 10 μM RT | 0.58 ± 0.04 | ND |
25 μM EGCG + 10 μM RT | 0.99 ± 0.04 | ND |
50 μM EGCG + 10 μM RT | 1.90 ± 0.03 | 0.5 ± 0.03* |
SH-SY5Y cells were treated with rotenone alone, EGCG alone or a combination of the two in serum-free and phenol-red free RPMI 1640 media. After 24 h of treatment, aliquots of media were analyzed for H2O2 by using the PeroXOquant Quantitative Peroxide Assay Kit (Pierce, Rockford, IL, USA). Each value represents the means ± SE of 4 experiments.
Significantly different from corresponding no pyruvate-containing media, P < 0.05, by Student’s t-test.