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. Author manuscript; available in PMC: 2008 Oct 24.
Published in final edited form as: Brain Res. 2007 Aug 22;1176:133–142. doi: 10.1016/j.brainres.2007.07.083

Table 1.

Effect of EGCG and rotenone on hydrogen peroxide content of culture media.

Treatment Hydrogen peroxide (μM)
No pyruvate media With 1.5 μM pyruvate media
Control ND ND
Rotenone
 1 μM ND ND
 5 μM ND ND
 10 μM ND ND
 25 μM ND ND
 50 μM ND ND
EGCG
 1 μM ND ND
 5 μM ND ND
 10 μM 0.33 ± 0.04 ND
 25 μM 0.72 ± 0.04 ND
 50 μM 1.72 ± 0.10 0.87 ± 0.03*
Rotenone (RT) + EGCG
 1 μM EGCG + 10 μM RT ND ND
 5 μM EGCG + 10 μM RT 0.30 ± 0.04 ND
 10 μM EGCG + 10 μM RT 0.58 ± 0.04 ND
 25 μM EGCG + 10 μM RT 0.99 ± 0.04 ND
 50 μM EGCG + 10 μM RT 1.90 ± 0.03 0.5 ± 0.03*

SH-SY5Y cells were treated with rotenone alone, EGCG alone or a combination of the two in serum-free and phenol-red free RPMI 1640 media. After 24 h of treatment, aliquots of media were analyzed for H2O2 by using the PeroXOquant Quantitative Peroxide Assay Kit (Pierce, Rockford, IL, USA). Each value represents the means ± SE of 4 experiments.

*

Significantly different from corresponding no pyruvate-containing media, P < 0.05, by Student’s t-test.