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. 2007 Dec 3;118(1):316–332. doi: 10.1172/JCI32752

Figure 2. Incubation of McA cells with OA or IL increases GRP78 mRNA and protein levels.

Figure 2

(A). Incubation of McA cells with either OA (0.4–1.6 mM) or IL (100–1,000 mg/dl) for 3 hours did not affect mRNA levels of GRP78. However, incubation for 6 or 16 hours with OA or IL increased GRP78 mRNA in a dose-dependent manner. Data are mean ± SD normalized to cells incubated without OA or IL (n = 6 for each condition); *P < 0.05, **P < 0.001 versus control incubations. (B) Incubation of McA cells for 3 or 6 hours with OA (0.4 mM) or IL (500 mg/dl) did not significantly affect GRP78 protein levels. (C) GRP78 protein was significantly increased by incubation with either OA (0.4 mM) or IL (500 mg/dl) for 16 hours. ER stress, as indicated by increased GRP78, was also induced by a 3-hour treatment with tunicamycin (5 μg/ml) as a positive control. Protein data are mean ± SD normalized to cells incubated without OA or IL (n = 3 for each condition); *P < 0.001 versus control incubations.