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. 2007 Dec 3;118(1):316–332. doi: 10.1172/JCI32752

Figure 3. OA or IL induces phosphorylation of eIF2α in McA cells.

Figure 3

(A) Incubation of McA cells for 3 or 6 hours with OA (0.4 mM) or IL (500 mg/dl) did not significantly induce phosphorylation of eIF2α. (B) Phosphorylation of eIF2α was significantly increased by incubation with either OA (0.4 mM) or IL (500 mg/dl) for 16 hours without a change in total eIF2α protein level. ER stress was also induced by a 3-hour treatment with tunicamycin (5 μg/ml) as a positive control. Lanes were run on the same gel but were noncontiguous. Data are mean ± SD normalized to cells incubated without OA or IL (n = 3 for each condition); *P < 0.01 versus control incubations.