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Coexpression of CD34 and HLA-DR by cells in a human cutaneous tissue scar. Skin specimens were fixed, sectioned, and labeled with both anti-CD34 mAb and anti-HLA-DR mAb. Binding was detected with an immunoperoxidase-linked (anti-CD34) and an alkaline phosphatase-linked (anti-HLA-DR) secondary antibody. The anti-CD34 positive areas appear brown and granular and the anti-HLA-DR positive regions stain red. The isotype control displays no immunoreactivity. [×200 (A); ×1,000 (B and C).]
