. 2007 Oct 18;7:91. doi: 10.1186/1471-2180-7-91

Table 2.

Primers, their target, sequence, primer pairings and predicted amplicon size of primer pair.

Target	Primer name	Sequence (5' to 3')	Paired primer set and predicted amplicon size
Conserved for each IS element	IS1111-1	ACTGCGTTGGGATACCCATC	NA^a
IS1	IS1	TAATGGGCGACCAAGTCGA	IS1111-1; 501 bp
IS2	IS2	GGCTGAATGAATGCCTTCCA	IS1111-1; 501 bp
IS3	IS3	AAAAAGACCCTTCAGGTAATGGAA	IS1111-1; 477 bp
IS4	IS4	TTCCGCCATGACCAACTTC	IS1111-1; 519 bp
IS5	IS5	GTCGGTCAACGTCGTCACAT	IS1111-1; 515 bp
	556F	AACCGTGGCTGAGCGATC	IS5; NA
IS6	IS6	CCATTTGCAATAGCCGTGAA	IS1111-1; 477 bp
IS7	IS7	GCGGTTTTGGTACAATACTGTCATT	IS1111-1; 571 bp
	1091F	TGCAGGACATATTGTTGATGTCAC	IS7; NA
IS8	IS8	GGCAAGGATGACGAATGGAT	IS1111-1; 471 bp
IS9	IS9	GCCTCAGCCGATTTCGAG	IS1111-1; 492 bp
	1219F	CCCGAAGGTATCCATAACTCTGG	IS9; NA
IS10	IS10	GTGCGGCATTCGTGGTAGA	IS1111-1; 481 bp
	1269R	GATTTCCATCGACGTCCGC	1273R; NA
	1273R	TTCAGTTTATAGCGGCAGGACC	IS1111-1; NA
IS11	IS11	AAAGCCGTTCCCTACCGTG	IS1111-1; 481 bp
IS12	IS12	ACGTAGTTTGGAAAGGATACCCG	IS1111-1; 482 bp
IS13	IS13	GATGAGCGTCCACCACTGAA	IS1111-1; 537 bp
IS14	IS14	TGCTACCAACAGACTTACGGCA	IS1111-1; 481 bp
	1701R	TAAACACGAGGCGCTAGGTCA	IS14; NA
IS15	IS15	AACGCCCATGTGAGAAACATC	IS1111-1; 531 bp
	1718F	ACGGGTGTTCAAGCAGCC	IS15; NA
IS16	IS16	ATGACTTCTGATAGGGACTGTGCTC	IS1111-1; 511 bp
	1761F	GCCGTTGATCTTGCCGATAA	IS16; NA
IS17	IS17	CGAATCGCATTGGCACAGT	IS1111-1; 477 bp
IS18	IS18	CGTTTGCTGCTATACGACATGAA	IS1111-1; 532 bp
IS19	IS19	GTCGCCCAGGAGTGTTTCTG	IS1111-1; 531 bp
	1960R	ACAAAGACGACTCAGTTCGGC	1963R; NA
	1963R	CGATGTCTGCCTTTAGGAGGTC	1960R; NA
	1963R-seq	GTAGAAAGAGTCTCTCTTG	NA
IS20	IS20	ACGTCAATTACATCGAGCATTCA	IS1111-1; 470 bp
	1987F	AGGGCCATCTTGACCTGGA	1993F; NA
	1993F	CGCACTGAGCGCATTGAC	1987F; NA

^aNot applicable