Table 2.
MINE-A, -B and -C expression relatively to housekeeping genes Ilv5 and Ef1
| Tissue | MINE-A | MINE-B | MINE-C | |||
| R.t.a (Ef1alpha) | R.t.a (Ilv5) | R.t.a (Ef1alpha) | R.t.a (Ilv5) | R.t.a (Ef1alpha) | R.t.a (Ilv5) | |
| mycelium | 4.4 ± 1.6 | 70.1 ± 14.5 | 4.4 ± 0.2 | 71.2 ± 5.1 | 1.4 ± 0.1 | 24.1 ± 8.5 |
| spore | 2.1 ± 0.4 | 20.0 ± 0.7 | 4.7 ± 0.5 | 44.6 ± 1.7 | 2.8 ± 0.3 | 26.4 ± 1.0 |
| infection 24 hours | 10.4 ± 1.0 | 154.3 ± 17.8 | 4.3 ± 1.24 | 64.4 ± 10.2 | 3.1 ± 0.6 | 42.7 ± 7.4 |
aRelative transcription, % of the corresponding housekeeping gene transcript level. To specifically amplify each individual chimeric element, a combination of primers q1+q2 was used (See Figure 3). Primer q1 is specifically designed to the WEIRD-MGL junction site of the desired MINE element. All quantitative RT-PCR experiments were performed in quadruplicate. Relative transcription values were calculated as described on the legend to the table 1.