Fig. 5.

Pre-stimulation of MLN cells with LA does not induce OVA-specific cytokine production markedly compared with pre-stimulation with BA. MLN cells were isolated from germ-free OVA23-3 mice. In the pre-stimulation group, these cells and intestinal bacteria were co-cultured for 24 h and then the supernatants were removed by centrifugation. OVA was added for a further stimulation of 48 h. In the simultaneous stimulation, MLN cells were co-cultured with intestinal bacteria and OVA for 48 h. Final concentration of intestinal bacteria (LA or BA) in the culture was 50 μg/ml, and OVA was added at 250 μg/ml. The supernatants were assayed for cytokines (IFN-γ, IL-10) by ELISA. All data represent mean ± S.D. Data shown are representative of 3 independent experiments