Fig. 2.

Effect of inositol derivatives on translocation of GLUT4 in L6 myotubes (a) and in skeletal muscles of rats ex vivo (b). Proteins contained in plasma membrane fraction were prepared from cells after treatment with inositol derivatives or insulin (in lane 1, basal; 2, insulin; 3, d-pinitol; 4, d-chiro-inositol; 5, l-chiro-inositol; 6, muco-inositol and 7, epi-inositol) were subjected to SDS-polyacrylamide gel electrophoresis, transferred to a PVDF membrane, and then GLUT4 was detected by immunoblotting as described in the text. The protein level of IRβ in the plasma membrane fraction was also detected and shown in each panel as the internal control