Figure 5.

Specific interaction between immobilized CIITA activation domain and TAFs. [³⁵S]Methionine-labeled Drosophila and human TAFs were incubated with glutathione-agarose beads bound to either the control GST protein or a GST–CIITA-(17–148) fusion protein. After extensive washings, the agarose beads were boiled in SDS/PAGE buffer and bound proteins were analyzed by SDS/PAGE and autoradiography. (a) Binding of the CIITA activation domain was tested with labeled dTAF_II40 (lanes 1–3) or hTAF_II32 (lanes 4–6). The interactions of the [³⁵S]methionine-translated proteins with GST (lanes 3 and 6) is insignificant compared with that with GST–CIITA (lanes 2 and 5). (b) Binding of CIITA activation domain to dTAF_II60 (lanes 1–3) and hTAF_II70 (lanes 4–6).