Figure 1.

Regions of RFX5 that activate transcription in the Raji human B cell line. A series of 5′ deletions of the RFX5 coding region were fused to the GAL4-(1–147) DNA binding domain. These plasmids were designed to sequentially remove putative functional domains detected in the primary structure of RFX5. RFX5-(34–615) is essentially full-length, RFX5-(194–615) removes the DNA-binding domain, RFX5-(318–615) removes the central region, and RFX5-(477–615) removes the proline-rich region. Numbers used are the numbers of amino acid residues deleted from the reported translation initiation codon. pBXGAL-VP expresses the GAL4 DNA binding domain fused to the acidic activation domain of VP16-(413–490). Cells were transfected with 10 μg of pG5EC alone as a control for background activity by this reporter construct. The autoradiograph presents data from independent duplicate transfections of acetylated chloramphenicol species separated by TLC used to measure reporter gene activity.