Figure 4.

Interaction between RFX5 and CIITA detected in yeast. Histograms depict β-galactosidase reporter gene activity in yeast extracts from cells transformed with RFX5–GAL4 fusion protein expression plasmids or cotransformation that included a plasmid for expression of CIITA fused to the GAL4 activation domain. All data represent the mean activity detected in triplicate transformants. Individual measurements were within 5% of each other. pGBT9 encodes the GAL4 DNA binding domain alone. pTD-1 used in the positive control encodes a fusion protein made up of amino acids 84–708 of simian virus 40 large tumor antigen and GAL4 activation domain and pVa3 encodes a fusion protein made up of GAL4 DNA binding domain and amino acids 72–350 of p53 with which the simian virus 40T antigen interacts.