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Identification of sTNF-R by Western blotting using affinity-purified human anti-sTNF-R antibodies. Following SDS/PAGE, the sTNF-R was transferred to nitrocellulose paper and incubated for 2 hr with affinity-purified antibodies (10 μg/ml), followed by horseradish peroxidase-conjugated second anti-human IgG.
