Figure 2.

(A) Siva cDNA sequence obtained from HeLa and human thymus cDNA libraries with the translated ORF (189 aa) is shown. The initiation codon is defined by the upstream in-frame stop codon (−42). The coding region is followed by a stop codon (568), a polyadenylylation signal (709–714). The sequence has been deposited in the GenBank under the accession number U2938. (B) A death domain (DD) homology region toward the amino terminus has been identified in Siva, and its homology to FADD and RIP DDs was determined with use of the multiple sequence alignment program clustal. The shaded regions show homology and the residues in boldface type depict identical residues conserved among the three regions. (C) Putative B-box domain of Siva. Homologous residues between Siva and other proteins in the B-box domain are shaded.