Figure 3.

Isolation of splenic B and T lymphocytes and detection of the transgene H chain in the purified lymphocyte populations. B and T lymphocytes from the spleen of DNA-inoculated mice were sorted and purified on a fluorescence-activated cell sorter at the times indicated. Lane 1, fragment amplified with the primers pCL/pCD (VDJ); lane 2, fragment amplified with the primers pSE/pNAD [(NANP)₃]; lane 3, fragment amplified with the primers pNEL/pNED (NESTED); lane 4, fragment amplified with the primers pβA1/pβA2 (β-actin).