Table 1.
Recovery of iodinated IgA
| Protein | TCA-precipitable cpm (104)
|
Recovered cpm precipitated (104)
|
||
|---|---|---|---|---|
| Intubated | Recovered (% of intubated) | DNS-BSASepharose | Anti-α + anti-κ | |
| dIgA | 343 | 24.6 (7.2) | 2.0 | 5.0 |
| sIgA | 320 | 52.0 (16.3) | 10.4 | 19.3 |
Two and a half hours after intubation of iodinated dIgA and sIgA into the stomach, mice were killed and the intestinal washes were collected. dIgA and sIgA in intestinal washes were precipitated with 10% TCA and with antibodies, as described in Materials and Methods.