Figure 2.

Expression of PSA transgene in the prostate of the PSA1 transgenics. Total RNA was isolated from the prostate of the nontransgenic and six PSA1 transgenic lines and the human prostatic cell line LNCaP, and RT-PCR was performed with PSA-specific primers and mouse and human β-actin primers. PSA PCR products were visualized by either ethidium bromide staining (A) or Southern blotting using an internal fragment as the probe (B), and β-actin PCR products were visualized by ethidium bromide staining (C). Lanes: a, nontransgenic; b, P1–2; c, P1–4; d, P1–6; e, P1–7; f, P1–8; g, P1–9; h, LNCaP.