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Effect of vaccination with the mutated ERK2-derived peptide 9m on the growth of CMS5 cells. Spleen cells pulsed with 9m, 9wt, or a control erbB2-derived peptide, or nonpulsed naive spleen cells were injected subcutaneously in BALB/c female mice, five mice per group, on day 0 and day 7. Mice were challenged with 1 × 106 CMS5a cells subcutaneously on day 14, and tumor growth was measured. From day 0 to day 25, 10 ng of IL-12 was injected intraperitoneally every other day.
