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Immunoblotting to show the specificity of anti-p27kip1 mAb SX18F7. SX18F7 can specifically recognize the 50-kDa GST-p27kip1 fusion protein (indicated by arrow GST–p27kip1) but not GST. In transfected Saos-2 cells, SX18F7 can specifically react with 27-kDa p27kip1 (indicated by arrow p27kip1) expressed from CMV–p27kip1 plasmid. It does not crossreact with p21waf1/cip1 overexpressed from CMV–p21waf1/cip1 plasmid.
