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. 1997 Jun 10;94(12):6380–6385. doi: 10.1073/pnas.94.12.6380

Figure 2.

Figure 2

(A) Immunoblotting analysis of p27kip1, cyclin D1, cdk4, cyclin E, and cdk2 expression in human breast cancer cell lines as indicated. The anti-p27kip1 mAb SX53G8 and anti-cyclin D1, anti-cdk4, anti-cyclin E, and anti-cdk2 rabbit polyclonal antibodies are described in Materials and Methods. Lane 1 is the extract from early passaged primary breast myoepithelial cells. Lanes 2–13 are the human breast tumor cell lines: ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B, SKBR5, SKBR7, CAMA, GI-101, and BT20, respectively. (B) Growth curve of six human breast cancer cell lines as indicated (Upper, CAL51, ZR75-1, MCF-7, SKBR7, GI-101, and BT20). (Lower) The p27kip1 protein level of the same group of human breast cancer cell lines is shown. The p27kip1 expression level in the normal primary myoepithelial and luminal epithelial cells is used as a normal control and is labeled as myoepithelial and luminal, respectively.