Table 1.
Gene | Sense primer (5′–3′) | Antisense primer (5′–3′) | Internal primers (5′–3′, 30-mers) | Size of cDNA, bp | Nucleotide no. | GeneBank accession no. |
---|---|---|---|---|---|---|
β-actin | TTGTAACCAACTGGGACGATATGG | GATCTTGATCTTCATGGTGCTAGG | GGTCAGGATCTTCATGAGGTAGTCTGTCAG | 764 | 1552–2991 | J00691J00691 |
CPT-I | TATGTGAGGATGCTGCTTCC | CTCGGAGAGCTAAGCTTGTC | ACTCTGGTTGGAATCTGACTGGGTGGGATT | 629 | 3094–3722 | L07736L07736 |
ACO | GCCCTCAGCTATGGTATTAC | AGGAACTGCTCTCACAATGC | GCCTGCACTTTCTTCAGCCATCTTCAACGA | 634 | 2891–3524 | J02752J02752 |
ACC | ACTCCAGGACAGCACAGATC | TCTGCCAGTCCAATTCTAGC | ATGACATCTCGGCCATCTGGATATTCAGGG | 535 | 4646–5180 | J03808J03808 |
GPAT | TGATCAGCCAGGAGCAGCTG | AGACAGTATGTGGCACTCTC | CCAAGGAGCACCAGCAATTCATCACCTTTC | 504 | 1827–2330 | M77003M77003 |
UCP-2 | AACAGTTCTACACCAAGGGC | AGCATGGTAAGGGCACAGTG | GTCATCTGTCATGAGGTTGGCTTTCAGGAG | 471 | 311–782 | U69135U69135 |