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. 1997 Jun 10;94(12):6397–6402. doi: 10.1073/pnas.94.12.6397

Figure 1.

Figure 1

Assembly of full-length PKD1 cDNA. Five cDNAs were recovered from cDNA libraries. Brain 15 clone (BRL Gene-Trapper brain library), ZE4, ZE9 delta (λ ZAP EXPRESS library from the 145.19 cell line), which contains the PKD1 locus, but does not include the homologs and ZEFB7, DASH 14 (fetal brain libraries constructed in λ ZAP EXPRESS and λ DASH). Clones pTEQ 6A and 94-3 were derived by RT-PCR from brain mRNA. Restriction endonuclease sites used to assemble seven individual cDNAs are indicated.