Figure 2.

In vitro expression of full-length and truncation constructs of polycystin. (Upper) Signal peptide (S), LRR, Ig-like (Ig-like) domains, and transmembrane regions (TM) are indicated with estimated molecular weight of truncations on the right. (Lower) Shown is an autoradiogram of SDS/PAGE of in vitro-translated [³⁵S]methionine-labeled polycystin products (indicated below). All translations were performed with transcripts generated by either T7 RNA polymerase (sense orientation in pcDNA3 vector) or SP6 RNA polymerase (antisense orientation), PKD1 SP6. Molecular weight standards are shown on the left (M_r × 10⁻³).