Skip to main content
NCBI home page
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1963 May 1;17(2):423–441. doi: 10.1083/jcb.17.2.423

AN ELECTRON MICROSCOPE STUDY OF THE DEVELOPMENT OF SV40 VIRUS

Nicole Granboulan 1, P Tournier 1, R Wicker 1, W Bernhard 1
PMCID: PMC2106205  PMID: 13963379

Abstract

Kidney cells, predominantly from Cercopithecus monkeys but also from baboons, were infected in vitro with the SV40 virus. The infectious cycle was studied with the electron microscope by means of thin sections of cells fixed from 3 hours up to 11 days after infection. The frequency of virus formation and various nuclear and cytoplasmic lesions in relation to the infection are described. The virus particles appear in the nucleus in close contact with the chromatin. In a small number of cells they have been observed as early as 10 to 12 hours after infection, but most often they appear 24 to 48 hours afterward. Their mean diameter is 33 mµ. They have no membrane and are frequently arranged as crystal-like structures. In addition to the appearance of virus, one observes various lesions in the nucleoplasm and particularly in the nucleolus, which shows an early hypertrophy and produces unusual, dense condensations in contact with the nucleolonema. The importance of these nucleolar lesions and the relationship between the SV40 virus and the polyoma, common wart, and Shope papilloma viruses are discussed.

Full Text

The Full Text of this article is available as a PDF (2.7 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALMEIDA J. D., HOWATSON A. F., WILLIAMS M. G. Electron microscope study of human warts; sites of virus production and nature of the inclusion bodies. J Invest Dermatol. 1962 Jun;38:337–345. [PubMed] [Google Scholar]
  2. BANFIELD W. G., DAWE C. J., BRINDLEY D. C. Intracellular and extracellular particles in tissue cultures inoculated with parotid-tumor agent (polyoma virus). J Natl Cancer Inst. 1959 Nov;23:1123–1135. [PubMed] [Google Scholar]
  3. BERNHARD W., TOURNIER P. Ultrastructural cytochemistry applied to the study of virus infection. Cold Spring Harb Symp Quant Biol. 1962;27:67–82. doi: 10.1101/sqb.1962.027.001.010. [DOI] [PubMed] [Google Scholar]
  4. BOIRON M., PAOLETTI C., THOMAS M., REBIERE J. P., BERNARD J. [Infectious desoxyribonucleic acid extracted from cultures of kidney cells of the baboon infected by SV 40 virus]. C R Hebd Seances Acad Sci. 1962 Mar 12;254:2097–2099. [PubMed] [Google Scholar]
  5. DULBECCO R., FREEMAN G. Plaque production by the polyoma virus. Virology. 1959 Jul;8(3):396–397. doi: 10.1016/0042-6822(59)90043-1. [DOI] [PubMed] [Google Scholar]
  6. Dimayorca G. A., Eddy B. E., Stewart S. E., Hunter W. S., Friend C., Bendich A. ISOLATION OF INFECTIOUS DEOXYRIBONUCLEIC ACID FROM SE POLYOMA-INFECTED TISSUE CULTURES. Proc Natl Acad Sci U S A. 1959 Dec;45(12):1805–1808. doi: 10.1073/pnas.45.12.1805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. EDDY B. E., BORMAN G. S., BERKELEY W. H., YOUNG R. D. Tumors induced in hamsters by injection of rhesus monkey kidney cell extracts. Proc Soc Exp Biol Med. 1961 May;107:191–197. doi: 10.3181/00379727-107-26576. [DOI] [PubMed] [Google Scholar]
  8. GAYLORD W. H., Jr, HSIUNG G. D. The vacuolating virus of monkeys. II. Virus morphology and intranuclear distribution with some histochemical observations. J Exp Med. 1961 Dec 1;114:987–996. doi: 10.1084/jem.114.6.987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GIRARDI A. J., SWEET B. H., SLOTNICK V. B., HILLEMAN M. R. Development of tumors in hamsters inoculated in the neonatal period with vacuolating virus, SV-40. Proc Soc Exp Biol Med. 1962 Mar;109:649–660. doi: 10.3181/00379727-109-27298. [DOI] [PubMed] [Google Scholar]
  10. HAGUENAU F., BONAR R. A., BEARD D., BEARD J. W. Ultrastructure of the rabbit papilloma virus. J Natl Cancer Inst. 1960 Apr;24:873–881. [PubMed] [Google Scholar]
  11. HINZ R. W., BARSKI G., BERNHARD W. An electron microscopic study of the development of the encephalomyocarditis (EMC) virus propagated in vitro. Exp Cell Res. 1962 Mar;26:571–586. doi: 10.1016/0014-4827(62)90163-5. [DOI] [PubMed] [Google Scholar]
  12. HOWATSON A. F., ALMEIDA J. D. An electron microscope study of polyoma virus in hamster kidney. J Biophys Biochem Cytol. 1960 Jul;7:753–760. doi: 10.1083/jcb.7.4.753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. HOWATSON A. F., ALMEIDA J. D. Observations on the fine structure of polyoma virus. J Biophys Biochem Cytol. 1960 Dec;8:828–834. doi: 10.1083/jcb.8.3.828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. HSIUNG G. D., GAYLORD W. H., Jr The vacuolating virus of monkeys. I. Isolation, growth characteristics, and inclusion body formation. J Exp Med. 1961 Dec 1;114:975–986. doi: 10.1084/jem.114.6.975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. HUXLEY H. E., ZUBAY G. Preferential staining of nucleic acid-containing structures for electron microscopy. J Biophys Biochem Cytol. 1961 Nov;11:273–296. doi: 10.1083/jcb.11.2.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LEDUC E. H., BERNHARD W. Electron microscope study of mouse liver infected by ectromelia virus. J Ultrastruct Res. 1962 Jun;6:466–488. doi: 10.1016/s0022-5320(62)80003-3. [DOI] [PubMed] [Google Scholar]
  17. MATTERN C. F. Polyoma and papilloma viruses: do they have 42 or 92 subunits? Science. 1962 Aug 24;137(3530):612–613. doi: 10.1126/science.137.3530.612. [DOI] [PubMed] [Google Scholar]
  18. MAYOR H. D., MELNICK J. L. Icosahedral models and viruses: a critical evaluation. Science. 1962 Aug 24;137(3530):613–615. doi: 10.1126/science.137.3530.613. [DOI] [PubMed] [Google Scholar]
  19. MELNICK J. L. Papova virus group. Science. 1962 Mar 30;135(3509):1128–1130. doi: 10.1126/science.135.3509.1128. [DOI] [PubMed] [Google Scholar]
  20. STONE R. S., SHOPE R. E., MOORE D. H. Electron microscope study of the development of the papilloma virus in the skin of the rabbit. J Exp Med. 1959 Oct 1;110:543–546. doi: 10.1084/jem.110.4.543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. SWEET B. H., HILLEMAN M. R. The vacuolating virus, S.V. 40. Proc Soc Exp Biol Med. 1960 Nov;105:420–427. doi: 10.3181/00379727-105-26128. [DOI] [PubMed] [Google Scholar]
  22. WILLIAMS M. G., HOWATSON A. F., ALMEIDA J. D. Morphological characterization of the virus of the human common wart (verruca vulgaris). Nature. 1961 Mar 18;189:895–897. doi: 10.1038/189895a0. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES