Table 1.
Surface expression and coreceptor function of CXCR4 mutants and chimeras
| Construct | Surface staining | Cell–cell fusion
|
||||
|---|---|---|---|---|---|---|
| BH8 | 89.6 | BK132 | RF | SBL6669 | ||
| CXCR4 | +*† | +++ | +++ | +++ | +++ | +++ |
| CXCR2 | +‡ | − | − | − | − | − |
| 2444 | +*‡ | +++ | +++ | +++ | + | ++ |
| 2444b | +‡ | ++ | ++ | + | − | ++ |
| 4244 | − | − | − | − | − | − |
| 4424 | − | − | − | − | − | − |
| 4442 | +*† | + | + | ++ | ++ | + |
| 4222 | +† | − | − | − | − | − |
| 2242 | +‡ | + | + | ± | − | ND |
| 2224 | − | − | − | − | − | − |
| 2442 | +*‡ | + | + | ± | − | ND |
| 2442b | − | − | − | − | − | − |
| 4422 | − | − | − | − | − | − |
| 2244 | +‡ | − | + | − | − | + |
| CXCR4-tail | +*† | +++ | +++ | ND | ND | ND |
| CXCR4-NAA | +* | + | + | ND | + | ND |
Surface expression after transient expression in QT6 cells was determined by flow cytometry staining with 12G5, rabbit serum to the CXCR4 amino terminus, or 10G2. Fusion activity relative to wild-type CXCR4 was determined as in Figs. 2, 3, 4, with 66–100% = +++; 33–65% = ++; 10–33% = +; 5–10% = ±; 0–5% = −. Fusion results for coreceptors that functioned were the average of at least three experiments. ND, not determined.
*
12G5 stained cells expressing the indicated receptor.
†
Rabbit serum to the CXCR4 amino terminus stained cells expressing the indicated receptor.
‡
10G2 stained cells expressing the indicated receptor.