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. 1967 Jan 1;32(1):89–112. doi: 10.1083/jcb.32.1.89

THE CIRCUMFUSION SYSTEM FOR MULTIPURPOSE CULTURE CHAMBERS

I. Introduction to the Mechanics, Techniques, and Basic Results of a 12-Chamber (In Vitro) Closed Circulatory System

George G Rose 1
PMCID: PMC2107086  PMID: 10976203

Abstract

A self-contained mechanical system for circulating nutrient fluid through 12 tissue culture chambers is described in detail. This system utilizes nonperforated cellophane membranes in the chambers which separate the circulating nutrient from the tissue culture environments. The nutrient, therefore, is dialyzed through the cellophane of each chamber; some cell products are retained in the microenvironment between the closely apposed cellophane and cover slip, whereas the other cell products move from chamber to chamber in the circulating nutrient. The resultant environmental conditions directed by the circumfusion systems are highly favorable for maintaining the differentiation of chick embryo tissues over protracted periods; a number of micrographs are shown.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BUCHSBAUM R., KUNTZ J. A. The effects of certain stimulants and depresants on individual fibroblasts in a perfusion chamber. Ann N Y Acad Sci. 1954 Nov 17;58(7):1303–1310. doi: 10.1111/j.1749-6632.1954.tb45910.x. [DOI] [PubMed] [Google Scholar]
  2. CHRISTIANSEN G. S., DANES B., ALLEN L., LEINFELDER P. J. A culture chamber for the continuous biochemical and morphological study of living cells in tissue culture. Exp Cell Res. 1953 Sep;5(1):10–15. doi: 10.1016/0014-4827(53)90089-5. [DOI] [PubMed] [Google Scholar]
  3. CONSTABLE F. L., MOFFAT M. A. A glass tissue culture chamber for use in time-lapse cinematography. J Clin Pathol. 1958 Sep;11(5):455–457. doi: 10.1136/jcp.11.5.455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CRUICKSHANK C. N., COOPER J. R., CONRAN M. B. A new tissue culture chamber. Exp Cell Res. 1959 Mar;16(3):695–698. doi: 10.1016/0014-4827(59)90141-7. [DOI] [PubMed] [Google Scholar]
  5. Folkman J., Cole P., Zimmerman S. Tumor behavior in isolated perfused organs: in vitro growth and metastases of biopsy material in rabbit thyroid and canine intestinal segment. Ann Surg. 1966 Sep;164(3):491–502. doi: 10.1097/00000658-196609000-00012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HU F. N., HOLMES S. G., POMERAT C. M., LIVINGOOD C. S., McCONNELL K. P. A method for studying the effect of P32 on living adult human epidermal cells in a perfusion chamber. Tex Rep Biol Med. 1951;9(4):739–748. [PubMed] [Google Scholar]
  7. KRUSE P. F., Jr, MYHR B. C., JOHNSON J. E., WHITE P. B. PERFUSION SYSTEM FOR REPLICATE MAMMALIAN CELL CULTURED IN T-60 FLASKS. J Natl Cancer Inst. 1963 Jul;31:109–123. [PubMed] [Google Scholar]
  8. Kruse P. F., Jr, Miedema E. Production and characterization of multiple-layered populations of animal cells. J Cell Biol. 1965 Nov;27(2):273–279. doi: 10.1083/jcb.27.2.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MORGAN W. D., DAWE C. J. An intermittent perfusion and gas exchange method for continuous cultivation of cells in the Rose chamber. J Natl Cancer Inst. 1961 Jan;26:133–149. [PubMed] [Google Scholar]
  10. RICHTER K. M., WOODWARD N. W., Jr A versatile type of perfusion chamber for long-term maintenance and direct microscopic observation of tissues in culture. Exp Cell Res. 1955 Dec;9(3):585–587. doi: 10.1016/0014-4827(55)90089-6. [DOI] [PubMed] [Google Scholar]
  11. ROSE G. G. An unusual behavior of strain HeLa cells in tissue culture. J Cell Biol. 1962 Apr;13:153–157. doi: 10.1083/jcb.13.1.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. ROSE G. G., CATTONI M., POMERAT C. M. OBSERVATIONS ON ORAL TUMORS IN VITRO. 2. KARYOBIOSIS IN STRAIN KB CELLS. J Dent Res. 1964 Jul-Aug;43:580–605. doi: 10.1177/00220345640430041301. [DOI] [PubMed] [Google Scholar]
  13. ROSE G. G., CATTONI M., POMERAT C. M. Observations on oral tumors in vitro. 1. Zeiosis versus the nucleus and endoplasmic reticulum in strain KB. J Dent Res. 1963 Jan-Feb;42:38–53. doi: 10.1177/00220345630420014401. [DOI] [PubMed] [Google Scholar]
  14. ROSE G. G. Microkinetospheres and VP satellites of pinocytic cells observed in tissue cultures of Gey's strain HeLa with phase contrast cinematographic techniques. J Biophys Biochem Cytol. 1957 Sep 25;3(5):697–704. doi: 10.1083/jcb.3.5.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. ROSE G. G. NUCLEAR FOLDS VERSUS INTRANUCLEAR INCLUSIONS IN TISSUE CULTURES. J R Microsc Soc. 1964 Dec;83:377–390. doi: 10.1111/j.1365-2818.1964.tb00555.x. [DOI] [PubMed] [Google Scholar]
  16. ROSE G. G. PHASE-CONTRAST MICROSCOPY IN LIVING CELLS. J R Microsc Soc. 1964 Jun;83:97–114. doi: 10.1111/j.1365-2818.1964.tb00517.x. [DOI] [PubMed] [Google Scholar]
  17. ROSE G. G., POMERAT C. M., SHINDLER T. O., TRUNNELL J. B. A cellophane-strip technique for culturing tissue in multipurpose culture chambers. J Biophys Biochem Cytol. 1958 Nov 25;4(6):761–764. doi: 10.1083/jcb.4.6.761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. ROSE G. G. Special uses of the multipurpose tissue culture chamber. Tex Rep Biol Med. 1957;15(2):310–312. [PubMed] [Google Scholar]
  19. ROSE G. G. TIME-LAPSE CINEMICROGRAPHY OF CELLS IN TISSUE CULTURE. Bull Johns Hopkins Hosp. 1965 Jan;116:33–68. [PubMed] [Google Scholar]
  20. ROSE G. G. The Golgi complex in living osteoblasts. J Biophys Biochem Cytol. 1961 Feb;9:463–478. doi: 10.1083/jcb.9.2.463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. ROSE G. G. The morphological diversity of Gey's strain HeLa after ten years in tissue culture. Tex Rep Biol Med. 1962;20:308–337. [PubMed] [Google Scholar]
  22. ROSE G. A separable and multipurpose tissue culture chamber. Tex Rep Biol Med. 1954;12(4):1074–1083. [PubMed] [Google Scholar]
  23. Rose G. G. Observations on oral tumors in vitro. 3. Morphologic properties and longevity of nonviral intranuclear inclusions. J Natl Cancer Inst. 1966 Apr;36(4):747–773. doi: 10.1093/jnci/36.4.747. [DOI] [PubMed] [Google Scholar]
  24. SCHWARTZ B. The design and performance of a perfusion system for the culture of the lens. Part II. Arch Ophthalmol. 1960 Apr;63:607–624. doi: 10.1001/archopht.1960.00950020609003. [DOI] [PubMed] [Google Scholar]

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