Figure 5.

Effects of leptin and CNTF on tis-11 expression in mouse hypothalamus. Groups of three ob/ob mice received intraperitoneal injections of vehicle (−), leptin (L, 100 μg), or DH-CNTF (D-C, 10 μg) and were sacrificed 1 hr later by cervical dislocation. (A) Relative expression levels of tis-11 and β-actin (as control), as determined by RT-PCR. (B) In situ hybridization on coronal brain sections from vehicle- or cytokine-treated mice, using a ³⁵S-labeled cRNA probe for murine tis-11. (×100.)