Skip to main content
NCBI home page
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1967 Oct 1;35(1):53–59. doi: 10.1083/jcb.35.1.53

THE EFFECT OF THYMIDINE ON THE DURATION OF G1 IN CHINESE HAMSTER CELLS

R A Tobey 1, E C Anderson 1, D F Petersen 1
PMCID: PMC2107100  PMID: 6061724

Abstract

The generation time of a Chinese hamster cell line was varied by the use of different lots of sera in the culture media. Analysis of the division waves following thymidine synchronization showed that lengthening of the generation time was a result of an increase in duration of the G1 phase and that thymidine treatment reduced the duration of G1 back to its minimum value.

Full Text

The Full Text of this article is available as a PDF (444.5 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHANOCK R. M., HAYFLICK L., BARILE M. F. Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO. Proc Natl Acad Sci U S A. 1962 Jan 15;48:41–49. doi: 10.1073/pnas.48.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Galavazi G., Bootsma D. Synchronization of mammalian cells in vitro by inhibition of the DNA synthesis. II. Population dynamics. Exp Cell Res. 1966 Feb;41(2):438–451. doi: 10.1016/s0014-4827(66)80150-7. [DOI] [PubMed] [Google Scholar]
  3. Galavazi G., Schenk H., Bootsma D. Synchronization of mammalian cells in vitro by inhibition of the DNA synthesis. I. Optimal conditions. Exp Cell Res. 1966 Feb;41(2):428–437. doi: 10.1016/s0014-4827(66)80149-0. [DOI] [PubMed] [Google Scholar]
  4. HAM R. G. An improved nutrient solution for diploid Chinese hamster and human cell lines. Exp Cell Res. 1963 Feb;29:515–526. doi: 10.1016/s0014-4827(63)80014-2. [DOI] [PubMed] [Google Scholar]
  5. Mayhew E. Cellular electrophoretic mobility and the mitotic cycle. J Gen Physiol. 1966 Mar;49(4):717–725. doi: 10.1085/jgp.49.4.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mueller G. C., Kajiwara K. Actinomycin D and p-fluorophenylalanine, inhibitors of nuclear replication in HeLa cells. Biochim Biophys Acta. 1966 Jun 22;119(3):557–565. doi: 10.1016/0005-2787(66)90132-8. [DOI] [PubMed] [Google Scholar]
  7. NEWTON A. A., WILDY P. Parasynchronous division of HeLa cells. Exp Cell Res. 1959 Mar;16(3):624–635. doi: 10.1016/0014-4827(59)90130-2. [DOI] [PubMed] [Google Scholar]
  8. PUCK T. T., SANDERS P., PETERSEN D. LIFE CYCLE ANALYSIS OF MAMMALIAN CELLS. II. CELLS FROM THE CHINESE HAMSTER OVARY GROWN IN SUSPENSION CULTURE. Biophys J. 1964 Nov;4:441–450. doi: 10.1016/s0006-3495(64)86794-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. PUCK T. T., STEFFEN J. LIFE CYCLE ANALYSIS OF MAMMALIAN CELLS. I. A METHOD FOR LOCALIZING METABOLIC EVENTS WITHIN THE LIFE CYCLE, AND ITS APPLICATION TO THE ACTION OF COLCEMIDE AND SUBLETHAL DOSES OF X-IRRADIATION. Biophys J. 1963 Sep;3:379–397. doi: 10.1016/s0006-3495(63)86828-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. PUCK T. T. STUDIES OF THE LIFE CYCLE OF MAMMALIAN CELLS. Cold Spring Harb Symp Quant Biol. 1964;29:167–176. doi: 10.1101/sqb.1964.029.01.021. [DOI] [PubMed] [Google Scholar]
  11. TERASIMA T., TOLMACH L. J. Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells. Exp Cell Res. 1963 Apr;30:344–362. doi: 10.1016/0014-4827(63)90306-9. [DOI] [PubMed] [Google Scholar]
  12. Tobey R. A., Anderson E. C., Petersen D. F. RNA stability and protein synthesis in relation to the division of mammalian cells. Proc Natl Acad Sci U S A. 1966 Nov;56(5):1520–1527. doi: 10.1073/pnas.56.5.1520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tobey R. A., Petersen D. F., Anderson E. C., Puck T. T. Life cycle analysis of mammalian cells. 3. The inhibition of division in Chinese hamster cells by puromycin and actinomycin. Biophys J. 1966 Sep;6(5):567–581. doi: 10.1016/s0006-3495(66)86678-x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Yang S. J., Hahn G. M., Bagshaw M. A. Chromosome aberrations induced by thymidine. Exp Cell Res. 1966 Apr;42(1):130–135. doi: 10.1016/0014-4827(66)90326-0. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES