Figure 2.

Sequence of the two putative human C/EBPɛ promoter regions. The Pβ region is the major promoter used in HL60 cells, because the majority of the clones (>60%) identified by RACE–PCR were initiated at this site. The transcriptional start sites of the C/EBPɛ gene were determined by 5′-RACE–PCR and sequence analysis of several independent clones. Large arrows indicate sequence ends of the majority of cDNA clones for each promoter region. Small arrows show ends of less frequent RACE–PCR clones. The translation initiation codon is shown in uppercase and bold characters. Purine stretches are boxed.