Figure 5.

Activation of G-CSF receptor promoter-luciferase reporter construct by C/EBPα and C/EBPɛ in cotransfected HeLa cells. The G-CSF receptor-luciferase wild type (WT) and mutant (Mut) have been described (24). Increasing amounts of expression plasmids for C/EBPɛ or 0.5 μg of C/EBPα were cotransfected with a constant amount (5 μg) of both G-CSF receptor-luciferase reporter plasmids and 1 μg of β-galactosidase (pCMVβ) reporter. The amount of transfected DNA was adjusted with vector DNA to 10 μg per dish. The values are presented as a percentage of fold transactivation over the reporter. The experiments were repeated at least four times.