Figure 4.

Chemical reactivity of diet-derived AGEs in human serum. After passing over a lysozyme-linked Sepharose column, serum fractions obtained prior to (pre-test) or after AGE-diet (post-test) (5 mg/ml) were incubated with ¹²⁵I-labeled fibronectin fragments (FN) (6 ng/ml) for up to 72 hr at 37°C in the presence or absence of aminoguanidine (AG). Human IgG (5 mg/ml) was used as control. High molecular weight complex (HMW) formation in the mixtures was determined by autoradiography (A), and by phosphorimage (PI) analysis (B). Data are expressed as fold increase in PI units/mg protein above the control value (IgG) and represent the mean of three identical experiments, each done in duplicate.