Abstract
A fine structure study of the phragmoplast and developing cell plate has been made on glutaraldehyde-osmium tetroxide-fixed, dividing, cultured cells of the liquid endosperm of Haemanthus katherinae Baker. The phragmoplast arises between the telophase nuclei, usually in association with a remnant strand of spindle elements, and consists of an accumulation of microtubules oriented at right angles to the plane of the future cell plate. The microtubules, which are 200–240 A in diameter, occur in small clusters spaced at approximately 0.2–0.3 µ intervals along the plate. Short interconnections interpreted as "cross-bridges" have been observed between individual microtubules. Within each cluster there is an electron-opaque zone about 0.3 µ in width which can be attributed in part to an overlap of microtubules from both sides of the plate and in part to a local accumulation of an amorphous electron-opaque material. During development these dense zones become aligned in a plane which itself defines the plane of the plate. Vesicles, commonly observed in long files, are derived from a cytoplasmic matrix rich in elements of the endoplasmic reticulum and sparse in dictyosomes. They aggregate between the clusters of microtubules and eventually coalesce to form the cell plate.
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Selected References
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- BAJER A. CINE MICROGRAPHIC ANALYSIS OF CELL PLATE FORMATION IN ENDOSPERM. Exp Cell Res. 1965 Feb;37:376–398. doi: 10.1016/0014-4827(65)90186-2. [DOI] [PubMed] [Google Scholar]
- Bajer A., Allen R. D. Role of phragmoplast filaments in cell-plate formation. J Cell Sci. 1966 Dec;1(4):455–462. doi: 10.1242/jcs.1.4.455. [DOI] [PubMed] [Google Scholar]
- Bajer A. Notes on ultrastructure and some properties of transport within the living mitotic spindle. J Cell Biol. 1967 Jun;33(3):713–720. doi: 10.1083/jcb.33.3.713. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brinkley B. R., Stubblefield E. The fine structure of the kinetochore of a mammalian cell in vitro. Chromosoma. 1966;19(1):28–43. doi: 10.1007/BF00332792. [DOI] [PubMed] [Google Scholar]
- Hepler P. K., Newcomb E. H. Fine structure of cell plate formation in the apical meristem of Phaseolus roots. J Ultrastruct Res. 1967 Aug 30;19(5):498–513. doi: 10.1016/s0022-5320(67)80076-5. [DOI] [PubMed] [Google Scholar]
- INOUE S., BAJER A. Birefringence in endosperm mitosis. Chromosoma. 1961;12:48–63. doi: 10.1007/BF00328913. [DOI] [PubMed] [Google Scholar]
- PORTER K. R., MACHADO R. D. Studies on the endoplasmic reticulum. IV. Its form and distribution during mitosis in cells of onion root tip. J Biophys Biochem Cytol. 1960 Feb;7:167–180. doi: 10.1083/jcb.7.1.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paweletz N. Zur Funktion des "Flemming-Körpers" bei der Teilung tierischer Zellen. Naturwissenschaften. 1967 Oct;54(20):533–535. doi: 10.1007/BF00627210. [DOI] [PubMed] [Google Scholar]
- Pickett-Heaps J. D. Further observations on the Golgi apparatus and its functions in cells of the wheat seedling. J Ultrastruct Res. 1967 May;18(3):287–303. doi: 10.1016/s0022-5320(67)80119-9. [DOI] [PubMed] [Google Scholar]
- Pickett-Heaps J. D., Northcote D. H. Cell division in the formation of the stomatal complex of the young leaves of wheat. J Cell Sci. 1966 Mar;1(1):121–128. doi: 10.1242/jcs.1.1.121. [DOI] [PubMed] [Google Scholar]
- Pickett-Heaps J. D., Northcote D. H. Organization of microtubules and endoplasmic reticulum during mitosis and cytokinesis in wheat meristems. J Cell Sci. 1966 Mar;1(1):109–120. doi: 10.1242/jcs.1.1.109. [DOI] [PubMed] [Google Scholar]
- REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stockinger L., Cireli E. Eine bisher unbekannte Art der Zentriolenvermehrung. Z Zellforsch Mikrosk Anat. 1965 Dec 10;68(5):733–740. [PubMed] [Google Scholar]
- WHALEY W. G., MOLLENHAUER H. H. The Golgi apparatus and cell plate formation--a postulate. J Cell Biol. 1963 Apr;17:216–221. doi: 10.1083/jcb.17.1.216. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Whaley W. G., Dauwalder M., Kephart J. E. The Golgi apparatus and an early stage in cell plate formation. J Ultrastruct Res. 1966 Apr;15(1):169–180. doi: 10.1016/s0022-5320(66)80102-8. [DOI] [PubMed] [Google Scholar]