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. 1997 Jun 10;94(12):6490–6495. doi: 10.1073/pnas.94.12.6490

Figure 5.

Figure 5

Representative recordings of single-channel currents from WT ρ1, mutated ρ1(T314A), and mutated ρ1(T314A/L317A) receptors. Recordings were performed on outside–out patches pulled from oocytes at a holding potential of −100 mV. (A) GABA evoked single-channel currents from WT ρ1 receptors. (B) Single-channel currents of mutated ρ1(T314A) receptors in the absence of ligand. (C) Single-channel currents of mutated ρ1(T314A/L317A) receptors in the absence of ligand (top four traces) or in the presence of 2 μM GABA (bottom four traces). Data were sampled at 10 kHz and filtered at 0.5 kHz. The first two and last two traces in A, and all four traces in B and C (Upper and Lower) represent continuous recordings.