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. 1997 Jun 10;94(12):6523–6528. doi: 10.1073/pnas.94.12.6523

Figure 3.

Figure 3

FACS analysis of phosphorothioate oligodeoxynucleotide blockade of mAb binding to cell-surface targets. Shown is the binding of anti-flk-1 mAbs 115 (A), 73 (B), and DC101 (C) to C441 cells preincubated with either 2 μM of the oligodeoxynucleotides SdC28, #2, #2T, G6T12, or 10 μg/ml heparin or without oligodeoxynucleotide. All incubations were performed at 4°C to prevent flk-1 receptor internalization. The specific preincubation conditions used for each mAb-binding experiment are indicated in the appropriate figure. Each graph represents the MFI of mAb binding to cells pretreated with oligodeoxynucleotide or heparin relative to antibody binding alone on cells preincubated without competitors (normalized to MFI = 1). Measurements obtained with the irrelevant control are subtracted as background fluorescence. Data are averages of the relative MFI (± SEM) from at least three independent experiments. With the exception of mAb 115 binding to #2 treated cells in A, a statistical analysis (t test) of the data showed that mAb binding to cells pretreated with either oligodeoxynucleotide or heparin were significantly different (P < 0.05) from their respective controls.