Abstract
Commercially available glycogens and dextrans can be used as biological particulate tracers in work on capillary permeability. These polysaccharides are well tolerated in intravenous injection and induce no vascular leakage when applied topically (cremaster test) in mice and in Wistar-Furth rats. The particles stain adequately with lead after aldehyde-OsO4 fixation in phosphate buffer and provide a relatively wide set of probes (∼45 A-300 A) for work on the large and small pore systems.
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Selected References
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- ANKIER S. I., WEST G. B., HARRIS J. M., LUSCOMBE D. K. REACTIVITY OF WISTAR RATS TO DEXTRAN. J Pharm Pharmacol. 1965 Feb;17:118–119. doi: 10.1111/j.2042-7158.1965.tb07624.x. [DOI] [PubMed] [Google Scholar]
- Bruns R. R., Palade G. E. Studies on blood capillaries. I. General organization of blood capillaries in muscle. J Cell Biol. 1968 May;37(2):244–276. doi: 10.1083/jcb.37.2.244. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coulter H. D. Rapid and improved methods fr embedding biological tissues in Epon 812 and Araldite 502. J Ultrastruct Res. 1967 Oct 31;20(5):346–355. doi: 10.1016/s0022-5320(67)80104-7. [DOI] [PubMed] [Google Scholar]
- DROCHMANS P. [Morphology of glycogen. Electron microscopic study of the negative stains of particulate glycogen]. J Ultrastruct Res. 1962 Apr;6:141–163. doi: 10.1016/s0022-5320(62)90050-3. [DOI] [PubMed] [Google Scholar]
- FRASCA J. M., PARKS V. R. A ROUTINE TECHNIQUE FOR DOUBLE-STAINING ULTRATHIN SECTIONS USING URANYL AND LEAD SALTS. J Cell Biol. 1965 Apr;25:157–161. doi: 10.1083/jcb.25.1.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Farquhar M. G., Palade G. E. Cell junctions in amphibian skin. J Cell Biol. 1965 Jul;26(1):263–291. doi: 10.1083/jcb.26.1.263. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fearn H. J., West G. B. Reactivity of rats to dextran. J Pharm Pharmacol. 1965 Dec;17(12):813–813. doi: 10.1111/j.2042-7158.1965.tb07611.x. [DOI] [PubMed] [Google Scholar]
- GROTTE G., JUHLIN L., SANDBERG N. Passage of solid spherical particles across the blood-lymph barrier. Acta Physiol Scand. 1960 Dec 30;50:287–293. doi: 10.1111/j.1748-1716.1960.tb00182.x. [DOI] [PubMed] [Google Scholar]
- GROTTE G. Passage of dextran molecules across the blood-lymph barrier. Acta Chir Scand Suppl. 1956;211:1–84. [PubMed] [Google Scholar]
- Goth A. Effect of drugs on mast cells. Adv Pharmacol. 1967;5:47–78. doi: 10.1016/s1054-3589(08)60654-7. [DOI] [PubMed] [Google Scholar]
- HARRIS J. M., WEST G. B. Rats resistant to the dextran anaphylactoid reaction. Br J Pharmacol Chemother. 1963 Jun;20:550–562. doi: 10.1111/j.1476-5381.1963.tb01492.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Karnovsky M. J. The ultrastructural basis of capillary permeability studied with peroxidase as a tracer. J Cell Biol. 1967 Oct;35(1):213–236. doi: 10.1083/jcb.35.1.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MAJNO G., PALADE G. E., SCHOEFL G. I. Studies on inflammation. II. The site of action of histamine and serotonin along the vascular tree: a topographic study. J Biophys Biochem Cytol. 1961 Dec;11:607–626. doi: 10.1083/jcb.11.3.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MOWRY R. W., MILLICAN R. C. A histochemical study of the distribution and fate of dextran in tissues of the mouse. Am J Pathol. 1953 May-Jun;29(3):523–545. [PMC free article] [PubMed] [Google Scholar]
- RENKIN E. M. TRANSPORT OF LARGE MOLECULES ACROSS CAPILLARY WALLS. Physiologist. 1964 Feb;60:13–28. [PubMed] [Google Scholar]
- RICHARDSON K. C., JARETT L., FINKE E. H. Embedding in epoxy resins for ultrathin sectioning in electron microscopy. Stain Technol. 1960 Nov;35:313–323. doi: 10.3109/10520296009114754. [DOI] [PubMed] [Google Scholar]
- SABATINI D. D., MILLER F., BARRNETT R. J. ALDEHYDE FIXATION FOR MORPHOLOGICAL AND ENZYME HISTOCHEMICAL STUDIES WITH THE ELECTRON MICROSCOPE. J Histochem Cytochem. 1964 Feb;12:57–71. doi: 10.1177/12.2.57. [DOI] [PubMed] [Google Scholar]
- VENABLE J. H., COGGESHALL R. A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY. J Cell Biol. 1965 May;25:407–408. doi: 10.1083/jcb.25.2.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol. 1958 Jul 25;4(4):475–478. doi: 10.1083/jcb.4.4.475. [DOI] [PMC free article] [PubMed] [Google Scholar]