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. 1997 Jun 10;94(12):6559–6564. doi: 10.1073/pnas.94.12.6559

Figure 3.

Figure 3

Identification of Zea mI in maize pollen extracts and its association with wall extension activity. (A) Coomassie-stained SDS/polyacrylamide gel of total proteins eluted from maize pollen. (B) Western blot of total proteins eluted from maize pollen, using rabbit polyclonal antibodies against cucumber “S1” (Cs-EXP1) expansin (refs. 7 and 14). (C) Western blot of total proteins eluted from maize pollen, using monoclonal antibody directed against a Lol pI fragment (site D) (4). (D) Fractionation of maize pollen protein on CM-Sepharose. (E) Coomassie-stained SDS/polyacrylamide gel of CM-Sepharose fraction F7. (F) Western blot of fraction f7 probed with monoclonal antibody against Lol pI. (G) Extension curve of heat-inactivated walls of maize silks treated (arrow) with purified Zea mI (fraction f 7) brought to pH 4.5 with sodium acetate buffer.