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. 1988 Mar;170(3):1405–1407. doi: 10.1128/jb.170.3.1405-1407.1988

New locus for exopolysaccharide overproduction in Escherichia coli K-12.

K Zinkewich-Péotti 1, J M Fraser 1
PMCID: PMC210927  PMID: 3277956

Abstract

A new locus for exopolysaccharide overproduction in Escherichia coli K-12 was mapped by insertion mutagenesis. A 66% linkage to serA, which is located at 62 min on the E. coli K-12 linkage map, was shown by P1 transduction. The polysaccharide produced by the mutant was isolated and was shown to be similar to colanic acid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker T. A., Howe M. M., Gross C. A. Mu dX, a derivative of Mu d1 (lac Apr) which makes stable lacZ fusions at high temperature. J Bacteriol. 1983 Nov;156(2):970–974. doi: 10.1128/jb.156.2.970-974.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bertino J. B., Stacey K. A. A suggested mechanism for the selective procedure for isolating thymine-requiring mutants of Escherichia coli. Biochem J. 1966 Nov;101(2):32C–33C. doi: 10.1042/bj1010032c. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Casadaban M. J., Cohen S. N. Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4530–4533. doi: 10.1073/pnas.76.9.4530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gottesman S., Trisler P., Torres-Cabassa A. Regulation of capsular polysaccharide synthesis in Escherichia coli K-12: characterization of three regulatory genes. J Bacteriol. 1985 Jun;162(3):1111–1119. doi: 10.1128/jb.162.3.1111-1119.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Maler T. A simple enzymatic method for the analysis of macromolecular fucose in biological materials. Anal Biochem. 1980 Mar 1;102(2):340–343. doi: 10.1016/0003-2697(80)90164-5. [DOI] [PubMed] [Google Scholar]
  6. ORSKOV I., ORSKOV F., JANN B., JANN K. ACIDIC POLYSACCHARIDE ANTIGENS OF A NEW TYPE FROM E. COLI CAPSULES. Nature. 1963 Oct 12;200:144–146. doi: 10.1038/200144a0. [DOI] [PubMed] [Google Scholar]
  7. Orskov I., Nyman K. Genetic mapping of the antigenic determinants of two polysaccharide K antigens, K10 and K54, in Escherichia coli. J Bacteriol. 1974 Oct;120(1):43–51. doi: 10.1128/jb.120.1.43-51.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Orskov I., Orskov F., Jann B., Jann K. Serology, chemistry, and genetics of O and K antigens of Escherichia coli. Bacteriol Rev. 1977 Sep;41(3):667–710. doi: 10.1128/br.41.3.667-710.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. SAPELLI R. V., GOEBEL W. F. THE CAPSULAR POLYSACCHARIDE OF A MUCOID VARIANT OF E. COLI K 12. Proc Natl Acad Sci U S A. 1964 Aug;52:265–271. doi: 10.1073/pnas.52.2.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Silver R. P., Vann W. F., Aaronson W. Genetic and molecular analyses of Escherichia coli K1 antigen genes. J Bacteriol. 1984 Feb;157(2):568–575. doi: 10.1128/jb.157.2.568-575.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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