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. 1998 Jul 21;95(15):8479–8484. doi: 10.1073/pnas.95.15.8479

Figure 4.

Figure 4

Expression at the RNA level of T. thermophila telomerase components during vegetative growth, starvation for 14 h, and conjugation for 2–24 h as determined by RT-PCR or Northern hybridization. (A) Tt_TERT mRNA, 20 PCR cycles. (B) Telomerase RNA subunit (TER), 13 PCR cycles. (C) U1 snRNA (49), a control for RNA level and RT-PCR efficiency in the different samples, 13 PCR cycles. (D) Northern hybridization analysis of telomerase RNA, probing for U2 snRNA as an internal standard. (E) Quantitation of TERT mRNA levels (dark columns), divided by the signal obtained for the U1 snRNA and then normalized so that the level in vegetative cells equals 1.0. Each column represents the mean of two separate PCRs, with error bars representing the range of values. Quantitation of telomerase RNA levels as determined by Northern hybridization (striped columns), normalized to U2 snRNA so that the value for vegetative cells equals 1.0. Each column represents the mean of four separate samples, with error bars representing the SEM. (Quantitation of the RT-PCR reaction shown in B gives a similar result, with only the vegetative sample showing a slight difference).