Figure 3.

Phosphorylation of EI-N and EI. Phosphorylation experiments were conducted as described in Materials and Methods with [³²P]PEP as the phosphoryl donor. Protein samples were subjected to SDS/PAGE (16, 17). (A) Coomassie stained gel. (B) Autoradiograph. Standard molecular mass markers are shown at each end of the gel. Incubation mixtures contained the following proteins: Lanes: 1, fusion G (4 μg) and EI-N (4 μg); 2, fusion G (4 μg); 3, fusion Xa (4 μg) and EI-N (4 μg); 4, fusion Xa (4 μg); 5, EI-C (0.4 μg) and EI-N (4 μg); 6, EI-C (0.4 μg); 7, EI (2 μg); 8, EI-N (4 μg).