Figure 4.

Effect of concentration of EI-C on phosphorylation of EI-N. The experimental conditions were the same as those used for Fig. 3 (see Materials and Methods) with the following proteins in the incubation mixtures: Lanes: 1, 2 μg EI; 2, 4 μg EI-N; 3, 4 μg EI-N (140 pmol) plus 0.4 μg EI-C (11 pmol); 4, 4 μg EI-N plus 0.2 μg EI-C; 5, 4 μg EI-N plus 0.1 μg EI-C. In the latter, the mol ratio EI-N/EI-C = 51.