Table 1.

Growth of E. coli BL21(DE3)ΔptsI transformants on mannitol

E. coli BL21(DE3)ΔptsI transformed with plasmids*	Proteins or protein fragments encoded by plasmids	Growth†
pSXEI-N + pMal::EI-C/G	EI-N + fusion protein G	+
pSXEI-N + pMal::EI-C/Xa	EI-N + fusion protein Xa	+
pSXEI-N + pMal-p2	EI-N + MalE	−
pSEXI-N + pET::Mal	EI-N + MalE	−
pSYX39 + pSRT7	Intact EI	+
pSYX39 + pMal::EI-C/G	Fusion protein G	−
pSYX39 + pMal::EI-C/Xa	Fusion protein Xa	−
pSYX39 + p9C	EI-N	−
pSYX39 + pMal-p2	MalE	−
pSYX39 + pET::Mal	MalE	−

^*Plasmids are described in Figs. 1 and 2. 

^†Inocula were spread on agar plates containing 1% mannitol in salts (M9) medium; + signifies the formation of colonies. No colonies were observed on plates marked −.