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. 1998 Jul 21;95(15):8508–8513. doi: 10.1073/pnas.95.15.8508

Figure 2.

Figure 2

Distribution of ɛC and uracil-DNA glycosylase activities after FPLC chromatography on Mono S FPLC HR 5/5 column. The proteins containing the ɛCDG activity (fraction IV) were loaded on a Mono S FPLC HR 5/5 column and eluted by a linear NaCl gradient. (A) 250 fmol of the ɛC-34/G (■) duplex oligonucleotides were incubated with 1 μl from each column fraction for 10 min in a 50-μl reaction mixture. (B) 250 fmol of the U-34/G (▴) duplex oligonucleotides were incubated with 1 μl from each column fraction for 30 min in a 50-μl reaction mixture. The products of the reaction was analyzed as described in Fig. 1 and quantified by using the PhosphorImager (model Storm 840). For details see Materials and Methods.