Table 2.
The influence of the base opposite to ɛC and/or uracil residues on the rates of excision by E. coli dsUDG and hTDG proteins
| Substrate | DNA glycosylase activity, %
|
|
|---|---|---|
| E. coli dsUDG | hTDG | |
| ɛC/G | 100 | 100 |
| ɛC/A | 46 | 26.5 |
| ɛC/T | 93 | 21 |
| ɛC/C | 61 | 71 |
| U/G | 100 | 100 |
| U/A | 1 | <0 |
| U/T | 84 | <0 |
| U/C | 94 | 2 |
The ɛC-34 mer and/or U-34 mer were annealed with the complementary 34-mer oligonucleotides to generate the following mismatches: ɛC/G, ɛC/A, ɛC/T, and ɛC/C and/or U/G, U/A, U/T, U/C. Enzymatic activity was measured as a function of time, and the initial velocities were determined. For details see Materials and Methods.