Skip to main content
. 1998 Jul 21;95(15):8514–8519. doi: 10.1073/pnas.95.15.8514

Figure 6.

Figure 6

Inhibition of RNA helicase activity by VA RNAs. (A) Helicase assays with poly (I)⋅poly (C)-agarose fraction 24. Lane 6 contained a standard reaction. VA RNAI or VA RNAII were present in the reactions displayed in lanes 7–10 at 7.6 ng/ml (lanes 7 and 9) and 22.8 ng/ml (lanes 8 and 10). Control reactions lacking fraction 24 or ATP (lanes 4 and 5) were also conducted. Lane 3 contains boiled dsRNA substrate. (B) Helicase assays with purified human RNA helicase A. Lane 4 contained a standard reaction. VA RNAI or VA RNAII were present at 3.8 ng/ml (lanes 5 and 7) and 11.4 ng/ml (lanes 6 and 8). RNA helicase was omitted from the reaction of lane 3. Lanes 1 and 2 of A and B contain 32P-labeled dsRNA and ssRNA markers, and their positions are indicated.