Table 2. Temporal analysis of mitochondrial control region data (1183 bp) for marine iguana populations from 11 islands.
| Island | Year | No. of Haplotypes | FST | 95% CI Hdsim (η) | p value | 95% CI Hdsim (θ) | p value |
| Española | 1993 | 6 | 0.04018* | 0.66154–0.93077 | 0.0415 | 0.42949–0.89615 | 0.2541 |
| 2004 | 5 | 0.27526–0.79794 | 0.4285 | 0.22202–0.85360 | 0.3224 | ||
| Fernandina | 1993 | 18 | 0.02869* | 0.68519–0.94444 | 0.81640 | 0.49206–0.91799 | 0.9701 |
| 2004 | 23 | 0.72626–0.94343 | 0.99100 | 0.53232–0.91313 | 0.9999 | ||
| Floreana | 1993 | 6 | 0.08466* | 0.62989–0.93103 | 0.0305 | 0.59080–0.92874 | 0.0474 |
| 2004 | 10 | 0.65977–0.93563 | 0.4210 | 0.62989–0.93563 | 0.4792 | ||
| Genovesa | 1991 | 5 | 0.03575 | 0.55942–0.90242 | 0.0840 | 0.53720–0.91208 | 0.0989 |
| 2004 | 4 | 0.56471–0.91092 | 0.0061 | 0.44202–0.90420 | 0.0213 | ||
| Isabela | 1993 | 6 | −0.00697 | 0.56615–0.91692 | 0.2007 | 0.52923–0.92615 | 0.2096 |
| 2004 | 5 | 0.52910–0.90212 | 0.0667 | 0.52116–0.92328 | 0.0663 | ||
| Marchena | 1993 | 4 | 0.44125* | 0.47291–0.88177 | 0.2020 | 0.50493–0.92118 | 0.1318 |
| 2004 | 2 | 0.44558–0.86224 | 0.0019 | 0.35969–0.88350 | 0.0096 | ||
| Pinta | 1993 | 4 | −0.00848 | 0.23171–0.78659 | 0.0908 | 0.09512–0.82805 | 0.1738 |
| 2004 | 5 | 0.27721–0.80189 | 0.1649 | 0.14441–0.84107 | 0.2207 | ||
| San Cristóbal | 1993 | 3 | −0.01050 | 0.33333–0.82366 | 0.4161 | 0.37419–0.89677 | 0.2141 |
| 2004 | 3 | 0.27903–0.80166 | 0.3789 | 0.34238–0.88235 | 0.1611 | ||
| Santa Cruz | 1991 | 1 | 0.00000 | - | - | - | - |
| 2004 | 1 | - | - | - | - | ||
| Santa Fé | 1991/93 | 5 | −0.01503 | 0.42492–0.86336 | 0.4256 | 0.45946–0.90390 | 0.2689 |
| 2004 | 4 | 0.38384–0.84242 | 0.4221 | 0.48586–0.98707 | 0.1707 | ||
| Santiago | 1993 | 3 | 0.04572 | 0.58468–0.91532 | 0.0237 | 0.69556–0.94758 | 0.0055 |
| 2004 | 2 | 0.55769–0.90000 | 0.0132 | 0.72692–0.94487 | 0.0005 |
Data from each island is separated by sampling year (1991/1993 or 2004). FST values represent differentiation between the same population at two time points. Significant FST values (p<0.05) are labelled with an asterisk. H-test simulations were performed in the program DnaSP using both the number of mutations across all sequences (η) and population parameter θ (see Material and Methods). The 95% confidence intervals (CI) of simulated Hd values are reported for both the η- and θ-based simulations. To the right of the 95% CI ranges are p values which signify the location of the observed Hd value on the simulated distribution. Populations where the observed Hd value falls within the lowest 5% of the simulated data have p values in bold. This signifies that a population has lower Hd than expected under a neutral coalescent model.