Table 3.

Gene-specific oligonucleotides for cloning and expression analyses of dog CEA family members

gene	oligonucleotide name	oligonucleotide sequence	location of primers (exon)	size of PCR product (bp)*
CEACAM1	cfCEACAM1-5i-f cfCEACAM1-3i-r	5'-CGGCAGCTGCTCTCACC 5'-CCAGCAGGACAGGTTGCAT	5'-UTR 3'-UTR	1624
	CEACAM1cf-5i CEACAM1cf-3i	5'-GTCATGAAGCTTCGGCAGCTGCTCTCACC 5'-GTCAGTTCTAGACCAGCAGGACAGGTTGCAT	5'-UTR 3'-UTR	1648
	cfCEACAM1TM-f cfCEACAM1C3-r	5'-AGTTCTGGCCTTCCACCTG 5'-GGATGAGGAGGCTGAAGTTG	TM domain Cyt 3	270
	cfCEACAM1N-f cfCEACAM1C3-r	5'-CATCACCCTGAACGACACTG 5'-GGATGAGGAGGCTGAAGTTG	N domain Cyt 3	1183
CEACAM23	cfaCC23-N-fo cfaCC23-T-re	5'-TCCAGAACATCACCCTGAGC 5'-GACACCCCAGGGAAGCTG	N domain TM domain	1008
	cfCEACAM1-5i-f cfaCC23-3-re	5'-CGGCAGCTGCTCTCACC 5'-GAGCAGTACCATGGGGAAAC	5'-UTR 3'-UTR	1616
CEACAM24	cfaCC24-N-fo cfaCC24-T-re	5'-GTAGTAGACACACGAGTAATTGT 5'-CACAAGCGCTACTCCAATCA	N domain TM domain	258
	cfCEACAM1-5i-f cfaCC24-3-re	5'-CGGCAGCTGCTCTCACC 5'-GAGGGTTCCAGGGTGAAACT	5'-UTR 3'-UTR	1069
CEACAM25	cfaCC25-N-fo cfaCC25-T-re	5'-AATCTGCCTGGGGATCTTCT 5'-AATACGCCAGGGTAGCCATG	N domain TM domain	352
	cfCEACAM1-5i-f cfaCC25-3-re	5'-CGGCAGCTGCTCTCACC 5'-GGAAGGAGGGTTCCAAGGT	5'-UTR 3'-UTR	807
CEACAM28 (CEACAM30)**	cfaCC28-N-fo cfaCC28-T-re	5'-TTTGAAACTGCACTAATACGG 5'-TTGTGTGGAGCAGGAGACA	N domain TM domain	703 (427)
	cfCEACAM1-5i-f cfaCC28-3-re	5'-CGGCAGCTGCTCTCACC 5'-GGAAGGAGAAGTGTCATGAGGT	5'-UTR 3'-UTR	1374 (1095)
CEACAM29	cfaCC29-N-fo cfaCC29-T-re	5'-ACATCACCGTGAATAATGCC 5'-CGAGTGCTAGTTCGAGCAGA	N domain TM domain	447
	cfCEACAM1-5i-f cfaCC29-3-re	5'-CGGCAGCTGCTCTCACC 5'-CAGAAGGTTACAGGGGCTCA	5'-UTR 3'-UTR	844

* Size of largest cloned or predicted (CEACAM29) splice variant is shown

** CEACAM30 cDNA were also detected using the CEACAM28 primers (size of PCR products in brackets)

Cyt, cytoplasmic domain; TM, transmembrane domain; UTR, untranslated region