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TnpA-mediated formation of DNA multimers in the presence of ligase. 32P-labeled DNA fragments (20 ng) containing (a–e) or lacking (f, the GC-rich region of Spm) TnpA binding sites were incubated with 120 ng of TnpA in the presence or absence of ligase for the time indicated, were deproteinized, were fractionated on a 1% agarose gel, and were detected by autoradiography.
