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The effect of binding site number on TnpA-mediated DNA fragment ligation. (a–c) DNA fragments containing TnpA binding sites were ligated for 10 min in the presence of increasing amounts of TnpA, were fractionated on a 1% agarose gel, and were detected by autoradiography. (d) The percentage of shifted DNA in a–c is plotted as a function of the TnpA amount.
